Spore strips for ethylene oxide sterilization processes consist of bacteria spores inoculated on filter paper strips of 6 x 38 mm and packaged in glassine envelopes. They are inoculated with B. atrophaeus (# 9372) 10^6 CFU/carrier according to EN ISO 11138-1 + 2 and USP (United States Pharmacopeia). Spore strips that confirm with the European Pharmacopeia (EP) are also available.
A microbiological laboratory with sterile work bench is required to aseptically transfer the spore strips into the growth medium after sterilization.
The growth medium for optimized growth of B. atrophaeus spore strips is also available from gke.